Amperometric Enzyme Electrodes

نویسندگان

  • E. J. Calvo
  • C. Danilowicz
چکیده

Neste trabalho de revisão são analisados os avanços mais recentes em eletrodos enzimáticos amperométricos dando ênfase particular aos biosensores baseados na Glucose Oxidase e na Horseradish Peroxidase. A intermediação redox através de mediadores artificiais solúveis ou ligados a polímeros é discutida em termos dos desenvolvimentos teóricos recentes e verificações experimentais. É analisada a dependência da resposta amperométrica com a concentração do substrato, do mediador e da enzima assim como com o potencial do eletrodo e a espessura do filme. São também avaliadas as possíveis aplicações destes sistemas em esquemas multi-enzimáticos.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of Different Functionalized CNTs for Development of Choline Amperometric Biosensor

Choline oxidase (ChOx) was chosen as a model enzyme for evaluating the performance of CNTs’ functional groups for development of enzyme electrodes. CNTs were functionalized with carboxylic acid, amine or amide groups. Carboxylic acid, amine and amide functionalized CNTs were obtained by acid treatment, ethylenediamine or tetraethylenepentamine chemically modification and ammonia plasma treatmen...

متن کامل

Analytical problems facing the development of electrochemical transducers for in vivo drug monitoring: an overview.

Even though significant progress has been made in the development of electrodes for the in vivo detection of catecholamines, oxygen, and various cations, there has been little research on the feasibility of continuously monitoring drugs in whole blood by electrochemical sensing devices. Electroanalytical problems associated with the development of such electrochemical transducers include the ne...

متن کامل

Design of Amperometric Biosensors and Biofuel Cells by the Reconstitution of Electrically Contacted Enzyme Electrodes

The electrical contacting of redox enzymes with electrodes is the most fundamental requirement for the development of amperometric biosensors and biofuel cell elements. For the effective electrical communication of redox enzymes with electrodes the use of electron relay units that transport the electrons between the enzyme redox center and the conducting surface is essential. Also, the structur...

متن کامل

Immobilized enzyme-single-wall carbon nanotube composites for amperometric glucose detection at a very low applied potential.

The behaviour of support electrodes modified with randomly dispersed single-wall carbon nanotube meshes containing adsorbed glucose oxidase with respect to amperometric glucose detection at a low potential is demonstrated.

متن کامل

Adomian Decomposition Approach to a Mathematical Modeling and Analysis of Potentiometric and Amperometric Enzyme Electrodes

A mathematical model to describe the concentration of profiles and flux for potentiometric and amperometric enzyme electrodes and of enzyme reactors has been developed. This model contains a non-linear term related to Micahlies-Menten kinetics. Analytical expressions pertaining to the substrate concentration and product concentration were reported for all values of parameters P S   , and  . ...

متن کامل

A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1998